Hormonal regulation of apolipoprotein AI

REVIEW Hormonal regulation of apolipoprotein AI

Apolipoprotein AI (apo AI) is the major protein component of the serum high-density lipoprotein (HDL) particles. The antiatherogenic properties of apo AI alone or as part of HDL and their inverse correlation with the incidence of coronary heart disease underlie the clinical importance of the protein. A detailed understanding of the mechanisms by which apo AI is regulated will help us develop ne...

Transcriptional regulation of the apolipoprotein AI gene.

Since the original description of the gene encoding apolipoprotein AI (apoAI) considerable progress has been made in identifying the regulatory regions and corresponding transcription factors that regulate its transcription in liver and intestinal cells. Nuclear receptors (in particular HNF-4) have emerged as the dominant (although by no means the exclusive) transcriptional activators that driv...

Serum Apolipoprotein Ai

-high Density Lipoprotein, Apolipoprotein AI, and Phospholipid in Mice Expressing the Human Phospholipid Transfer Protein and Human Apolipoprotein AI Transgenes

Human plasma phospholipid transfer protein (PLTP) circulates bound to high density lipoprotein (HDL) and mediates both net transfer and exchange of phospholipids between different lipoproteins. However, its overall function in lipoprotein metabolism is unknown. To assess the effects of increased plasma levels of PLTP, human PLTP transgenic mice were established using the human PLTP gene driven ...

Quantification of human serum apolipoprotein AI by enzyme immunoassay.

We developed a quantitative assay for apolipoprotein AI (apo AI) in human serum, using a "sandwich"-type enzyme-linked immunosorbent assay. Diluted serum samples were pipetted into the wells of polystyrene microtiter plates that had been previously coated with purified rabbit anti-human apo AI antibodies. After incubation for 2 h and washing, antibodies conjugated to horseradish peroxidase (EC ...